SPIFE ImmunoFix

SPIFE ImmunoFix Kits

Cat. No.	Item	Description
3408	SPIFE ImmunoFix-15 (150 Profiles)	15 samples x 6 channels
3409	SPIFE ImmunoFix- 9 (90 Profiles)	9 samples x 6 channels
3401	SPIFE ImmunoFix- 6 (60 Profiles)	6 samples x 6 channels
3406	SPIFE ImmunoFix- 3 (30 Profiles)	3 samples x 6 channels

SPIFE ImmunoFix Kits include: 10 Gels, Acid Violet Stain, Blotters, Tris-Buffered Saline, Citric Acid Destain, Applicator Blades, Blotter Combs, Applicator Swabs, Fixative, and 1 vial each of Antisera to IgG, IgA, IgM, Kappa and Lambda.
NOTE: SPIFE ImmunoFix Gels and Antisera may be purchased separately:

Cat. No.	Item
3413	SPIFE IFE Antisera Kit (3 mL ea Fixative, IgG, IgA, IgM, K, λ)
3465	SPIFE IFE Light Chain Antisera Kit (3 mL ea Fixative, K, λ)
3466	SPIFE IFE-3 Gels (10 gels w/ consumables, no antisera)
3411	SPIFE IFE-6 Gels (10 gels w/ consumables, no antisera)
3469	SPIFE IFE-9 Gels (10 gels w/ consumables, no antisera)

 

QuickGel IFE Kit for SPIFE

Cat. No.	Item
3351	QuickGel IFE Kit - SPIFE

Kits include: 10 QuickGel IFE Gels, Acid Violet Stain, Tris-Buffered Saline, Citric Acid Destain, QuickGel Blotters, QuickGel IFE Blotter Combs, 20 Applicator Blades and 1 vial each Fixative, IgG, IgA, IgM, Kappa and Lambda antisera.
Additional Supplies and Equipment for QuickGel IFE SPIFE Procedure: REP Prep (Cat. No. 3100), SPIFE QuickGel IFE Cup Tray (Cat. No. 3371), SPIFE IFE 9/15 Dispo Cups (Cat. No. 3363), QuickGel Gel Block Remover (Cat. No. 1262), SPIFE QuickGel IFE Antisera Template (Cat. No. 3372), SPIFE QuickGel Electrodes (Cat. No. 1111), SPIFE QuickGel Gel Holder (Cat. No. 3358), SPIFE QuickGel IFE Chamber Alignment Guide (Cat. No. 3373).

 

Additional Supplies and Equipment for SPIFE ImmunoFix-15

Cat. No.	Item
1122	SPIFE IFE Multi-Channel Pipette
3356	Tip Spacers for SPIFE IFE-15 Multi-Channel Pipette (3/pkg)
3355	Tips for IFE-15 Multi-Channel Pipette
3407	SPIFE Urine IFE-15 Alignment Guide
3352	SPIFE Rigid Antisera Template-15
3362	SPIFE 3000 IFE-15 Dispo Cup Tray
3363	SPIFE IFE-9/15 Dispo Cups
3394	SPIFE IFE-9 Antisera Tray

Additional Supplies and Equipment for SPIFE ImmunoFix-9

Cat. No.	Item
1122	SPIFE IFE Multi-Channel Pipette
3396	Tip Spacers for SPIFE IFE-9 Multi-Channel Pipette (3/pkg)
3397	Pipette Tips for IFE-9 Pipettor
3392	IFE-9 Antisera Template
3394	IFE-9 Antisera Tray
3378	SPIFE IFE-9 Dispo Cup Tray
3363	SPIFE IFE-9/15 Dispo Cups
3391	SPIFE Urine IFE-9 Accessories (50 templates and blotters)

Additional Supplies and Equipment for SPIFE ImmunoFix-6 or -3

Cat. No.	Item
1122	SPIFE IFE Multi-Channel Pipette
3349	Tip Spacers for SPIFE IFE-6/3 Multi-Channel Pipette (3/pkg)
3355	Tips for IFE-15 Multi-Channel Pipette
3410	IFE-6 Antisera Template
3395	IFE-3 Antisera Template
1119	IFE Antisera Tray
3390	SPIFE Urine IFE-3/6 Accessories (50 templates and blotters)

Additional Supplies and Equipment for SPIFE ImmunoFix Procedures: REP Prep (Cat. No. 3100), Gel Block Remover (Cat. No. 1115).

Cat. No.	Item
3402	Pipettor Tips
3377	SPIFE 2000/3000 IFE 3/6 Dispo Cup Tray
3368	SPIFE IFE 3/6 Dispo Cups

• Additional Antisera for use with SPIFE ImmunoFix

  Cat. No.	Item	Description
  9409	IFE Antiserum to IgD	1 x 1 mL
  9410	IFE Antiserum to IgE	1 x 1 mL
  9412	IFE Free Kappa Antiserum	1 x 1 mL
  9413	IFE Free Lambda Antiserum	1 x 1 mL

  
  When performing IFE, if a reaction occurs with Kappa or Lambda but there's no reaction with IgG, IgA or IgM heavy chains, the sample should be tested with IgD and IgE antisera. A reaction indicates an IgD or IgE monoclonal gammopathy. A light chain reaction with no IgD or IgE reaction indicates Free Light Chain Disease.

  For use with serum or urine, the Free Kappa and Lambda Antisera are specific for free kappa and free lambda light chains respectively, reacting only with free light chains not attached to the heavy chain portion of the molecule.

ImmunoFix Quality Control

Cat. No.	Item	Description
9400	ImmunoFix Control Kit	1 x 0.5 mL IgG Kappa Control
		1 x 0.5 mL IgA Lambda Control
		1 x 0.5 mL IgM Control

ImmunoFix Controls are used to confirm antisera reactivity. After electrophoresis, the appropriate control is applied to a well at the end of each channel on the gel. Precipitin rings form around wells, indicating proper antigen/antibody reactions. Controls can also be treated as a patient sample to verify all phases of IFE procedure.